Pollinations were carried out in the greenhouse using emasculated flowers. Genetics of Potato Solanum tuberosum. The number of chromosomes observed among BC 2 plants was highly varied, ranging from 48 to The production of 5 x BC 1 progenies also confirmed the importance of 2 n gametes in polyploidization and gene flow during evolution. Mitotic analysis of root tip chromosomes indicated that most plants were pentaploid, rather than aneuploids. Indirect germplasm transfer through bridge species also has been accomplished by Adiwilaga and Brown 24 in the 4 x -2EBN species S.
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Alternatively, Tbr-4 x can be crossed with hybrids between wild species and Tbr haploids. Accepted Aug Mitotic analysis of root tip chromosomes indicated that most plants were pentaploid, rather than aneuploids.
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The triploid F 1 hybrids we produced flowered profusely and had a high pollen stainability, but they did not produce 2 n pollen Author information Article notes Copyright and License information Disclaimer. To sexually overcome these crossing barriers in the diploid species S.
Cross combination Genotypes analyzed, no. Ehlenfeldt and Hanneman 23 and Masuelli et al. Support Center Support Center. However, crosses between certain chromosome species and Tbr repeatedly fail due to strong sexual isolating mechanisms.
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Somatic hybridization has been applied successfully, and tetraploid or hexaploid hybrids have been obtained between several 1EBN diploid species, including S. Plant Breeding in the s. The production of near-pentaploid hybrids probably is due to the loss of chromosomes during megasporogenesis in the 3 x female parent, perhaps by occasional omission from a restitution nucleus.
The reaction conditions were as described by Williams et al.
The development of the EBN hypothesis led to the suggestion that in vivo EBN manipulations might be an efficient, simple, and reproducible approach for exploiting isolated germplasm 712 The number of chromosomes observed among BC 2 plants was highly varied, rm from 48 to Inter-EBN introgression by in vivo manipulations was used to transfer genes from diploid S.
M, molecular weight marker kb. Peloquin S J, Ortiz R. Plant Mol Biol Rep.
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Indeed, 2 n gametes from 3 x plants are balanced functional gametes that lead to a compatible EBN ratio 2: All derived plants were vigorous and tuberized under long day conditions. Genetics of Potato Solanum tuberosum. Rapid progress in obtaining a large number of chromosome genotypes is expected after 10 or two further backcross generations, and the availability of Cmm-specific RAPDs could speed up the selection of tetraploid Cmm-Tbr hybrids.
Lower somatic chromosome numbers were predominant, manifesting a trend toward tetraploidy. Resulting seeds were treated with gibberellic acid 1, ppm for 24 hr to break dormancy and sown in Styrofoam trays, and the seedlings obtained were transplanted to pots. As a result, their EBN is four, indicating no endosperm barriers to the introgression of genes from Cmm.
The results clearly demonstrate the feasibility of germplasm introgression from sexually isolated diploid 1EBN species into the 4 x 4EBN gene pool of the cultivated potato using sexual hybridization.
Root tips were collected for mitotic analysis to check the ploidy level. Murashige T, Skoog F. A tetraploid clone of Cmm PI Cmm-4 x was produced by in vitro tissue culture of leaf explants The in vivo direct method we used also could be applied to exploit sexually isolated relatives of other species where an EBN system is operating.
In any case, the possibility of using pentaploids both as male and female parents will allow derivation of progenies with either Cmm or Tbr cytoplasm.
This paper is contribution 1150. This doubling technique already has been used with other Solanum species and exploits the somaclonal variation induced by the tissue culture conditions